TB-500¶

Tags: Tissue Repair, Regenerative, Systemic Healing

Quick Facts¶

• Typical Dose: 2-5mg
• Frequency: 2-3x/week
• Route: Injectable (SubQ or IM) — belly, thigh, shoulder, or larger muscles
• Cycle: 6-8 weeks (typical)
• Storage: 2-6°C (refrigerated)

Overview¶

TB-500 is a synthetic 7-amino acid fragment (Ac-LKKTETQ) corresponding to the active actin-binding region (positions 17-23) of thymosin beta-4. Originally developed for veterinary use in horses, this fragment retains tissue repair and anti-inflammatory properties while being more stable than the full peptide. Banned by WADA for its performance-enhancing effects.

Key Benefits¶

• Superior systemic tissue repair
• Accelerated healing of muscles, tendons, ligaments, and organs
• Enhanced cell migration and angiogenesis for regenerative effects

Mechanism of Action¶

Injectable TB-500 provides systemic distribution for whole-body tissue repair. Acts on actin dynamics to regulate cell migration, promote angiogenesis, reduce inflammation, and activate stem cell differentiation to accelerate healing.

Molecular Information¶

• Weight: 4,963.44 Da
• Length: 43 amino acids
• Type: Thymosin fragment

Amino Acid Sequence:

Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser

N-terminal acetylation for enhanced stability

Research Protocols¶

Timing: TB-500 timing is flexible and doesn't require an empty stomach. For localized injuries, inject as close to the injury site as possible. For systemic effects, standard subcutaneous injection sites work well.

How to Reconstitute¶

1. Clean work area and hands thoroughly with alcohol.
2. Calculate required bacteriostatic water (BAC) volume using a calculator.
3. Draw calculated BAC water slowly into syringe.
4. Inject BAC water slowly down the side of the vial (never directly onto the powder).
5. Gently swirl vial until powder completely dissolves (never shake vigorously).
6. Store reconstituted solution in refrigerator and use within 28 days.

Important: Always use bacteriostatic water (BAC). Sterile technique is essential.

Interactions¶

!!! info "Compatible" - BPC-157 — Synergistic - CJC-1295 — Synergistic - IGF-1 — Synergistic - Ipamorelin — Compatible - GHRP-6 — Compatible - Melanotan II — Compatible

!!! warning "Monitor" - Immunosuppressive medications — Consult provider

!!! danger "Avoid" - Active cancer treatment - Competitive sport (WADA prohibited)

Quality Indicators¶

!!! success - White to off-white, fine lyophilized powder evenly distributed in vial with light, fluffy texture - Clear, colorless solution after reconstitution with no visible particles or cloudiness - Intact vacuum seal with secure rubber stopper showing no contamination - Minor powder clumping acceptable if dissolves completely with gentle swirling

!!! danger - Discolored powder (yellow, brown) or crystalline appearance indicating degradation or contamination - Persistent cloudiness, floating particles, or precipitation after reconstitution - Broken vacuum seal or damaged vial - Vigorous shaking during reconstitution

What to Expect¶

• Week 1-2: Reduced inflammation and improved tissue sensation
• Week 2-4: Accelerated healing rate and improved tissue quality
• Week 4-8: Maximum tissue repair and regenerative benefits
• Week 6-12: Continued improvement in tissue strength and function
• Side effects: Generally minimal, possible injection site reactions
• Most effective for: Muscle, tendon, ligament, and soft tissue injuries

Side Effects & Safety¶

• Generally well tolerated with excellent safety profile in clinical data
• Use sterile injection technique to prevent infection
• Mild injection site reactions are normal and temporary
• No known serious adverse effects in clinical studies
• Consult healthcare provider if on immunosuppressive medications
• Not recommended during active cancer treatment
• Avoid during pregnancy or breastfeeding
• WADA prohibited - not for competitive athletes

References¶

!!! quote - Human Phase 2 | Topical application | Accelerated healing rate

Dermal Wound Healing Study (2012)

TB-500 demonstrated significant acceleration in wound healing rates in patients with chronic pressure ulcers and venous stasis ulcers compared to placebo controls.

!!! quote - Human Clinical Trial | IV administration | Improved cardiac function

Cardiac Tissue Regeneration (2016)

Pilot study showed TB-500 pre-treated endothelial progenitor cell transplantation improved left ventricular function by >50% in post-MI patients.

!!! quote - Mouse Model | 6 months | Enhanced skeletal muscle repair

Muscle Fiber Regeneration (2015)

TB-500 improved skeletal muscle fiber regeneration in dystrophin-deficient mice, showing potential for muscular dystrophy treatment.