Glow Protocol¶
Tags: Glow, Combination Peptide, BPC-157, TB-500, GHK-Cu, Skin Rejuvenation
Quick Summary
The Glow Protocol is a combination peptide injection containing BPC-157, TB-500, and GHK-Cu marketed for skin rejuvenation and anti-aging. Careful dosing is required to ensure individual peptides remain within therapeutic ranges.
Quick Facts¶
- Typical Dose: Varies by formulation — calculate individual peptide amounts
- Route: Injectable (subcutaneous) — abdominal area, outer thigh, upper arm
- Cycle: 8–12 weeks (monitor accumulation and individual peptide requirements)
- Storage: 2–8°C — refrigerate, protect from light
Overview¶
The Glow Protocol is a combination injection containing BPC-157, TB-500, and GHK-Cu (copper peptide) marketed for skin rejuvenation and anti-aging. Individual components have supporting research, but combination products require careful dosing to avoid delivering individual peptides outside their therapeutic ranges.
Key Benefits¶
- Combination delivery of three regenerative peptides in a single injection
- Targets wound healing, angiogenesis, and collagen stimulation
Mechanism of action¶
- BPC-157: anti-inflammatory and tissue repair effects
- TB-500: supports angiogenesis and tissue remodeling
- GHK-Cu: stimulates collagen production and skin repair
Research indications¶
- Skin rejuvenation — most effective
- Wound healing — effective in many contexts
- Dosing concerns — moderate; individual responses vary with formulation
Research protocols¶
| Goal | Dose | Frequency | Route |
|---|---|---|---|
| Conservative approach | Calculate individual peptide amounts | Based on component requirements | Subcutaneous injection |
| Standard protocol | Per supplier guidelines | As recommended | Subcutaneous injection |
| Individual peptide alternative | Separate peptides | Optimized schedules | Multiple injections |
Timing note: consider each peptide's half-life and optimal schedule when planning combination dosing.
Interactions¶
Compatible / Complementary
- NAD+ precursors — Generally compatible
- Vitamin C — Generally compatible
Monitor
- Retinoids — Timing required
- AHA/BHA — Timing required
- Individual GHK-Cu dosing — Monitor for copper accumulation
- Individual BPC-157 dosing — May interact
Avoid
- Some combinations with individual GHK-Cu and BPC-157 should be avoided — consult healthcare provider
How to reconstitute¶
- Verify vial contents and individual peptide amounts before reconstitution.
- Calculate the target concentration based on each peptide's therapeutic dose.
- Allow vial and bacteriostatic water to reach room temperature before mixing.
- Clean vial tops with alcohol swabs and let air dry completely.
- Add the calculated volume of bacteriostatic water for the desired concentration.
- Insert the needle at an angle and inject the water slowly down the vial wall to avoid foaming.
- Gently swirl until fully dissolved (do not shake vigorously).
- Label the vial with reconstitution date, total volume, and calculated individual peptide concentrations.
- Store refrigerated at 2–8°C and use within 28 days.
Quality Indicators¶
Positive Signs
- Clear solution when properly reconstituted; no particles
- Vial lists individual peptide amounts (not just total weight)
- Temporary blue-green copper discoloration at injection sites is normal
Warning Signs
- Individual peptide amounts exceed recommended therapeutic ranges — do not use
- Cloudiness or particles present
What to expect¶
- Individual responses vary significantly based on peptide ratios in a formulation
- Effects are primarily from individual component peptides rather than guaranteed synergy
- May require dose adjustments based on tolerance to each peptide
- Consider using individual peptides if combination ratios are not optimal for you
Side effects & safety¶
- CRITICAL: calculate individual peptide doses before starting any combination protocol
- Combination ratios can exceed therapeutic ranges for some peptides — monitor carefully
- Monitor for copper accumulation effects (temporary skin discoloration)
- Start with minimal doses to assess tolerance and consult a healthcare provider experienced with peptide dosing
References¶
BPC-157 Tissue Repair Mechanisms (2024)
Research on BPC-157's role in tissue repair and anti-inflammatory effects.
GHK-Cu Anti-Aging Research (2023)
Examination of GHK-Cu's anti-aging and collagen synthesis properties.
TB-500 Angiogenesis Studies (2024)
Investigation of TB-500's role in tissue remodeling and angiogenesis.