DSIP¶

Tags: DSIP, Sleep, Neuromodulator

Overview¶

DSIP (Delta Sleep-Inducing Peptide) is a naturally occurring nonapeptide (Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu) first isolated in 1974 from rabbit brain. Originally identified for sleep-promoting properties, DSIP has since been investigated for broader neuromodulatory effects including stress reduction, pain modulation, and endocrine regulation. Clinical efficacy and mechanisms remain under active research.

Key Benefits¶

• Promotes deep (delta) slow-wave sleep
• Reduces sleep latency and nocturnal awakenings without morning grogginess
• May reduce stress and cortisol through HPA-axis modulation
• Potential pain-relief effects in some clinical reports

Mechanism of Action¶

DSIP appears to modulate multiple neurotransmitter systems (GABA, NMDA, endogenous opioid pathways) and influence the hypothalamic–pituitary–adrenal axis, supporting natural sleep architecture rather than producing sedation.

Molecular Information¶

• Type: Nonapeptide
• Sequence: Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu
• Length: 9 amino acids

Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu

Research Indications¶

• Sleep: deep-sleep enhancement and improved sleep efficiency (Most Effective)
• Mood: anxiety and mood stabilization (Effective)
• Chronic pain: reported benefit in small clinical series
• Stress management and withdrawal support
• Athletic recovery (short-term protocols)

Research Protocols¶

Timing: Best taken 30–60 minutes before intended bedtime; some prefer earlier evening dosing (2–3 hours) for gradual onset. Consistent timing improves outcomes.

Interactions¶

How to Reconstitute¶

1. Clean vial tops with an alcohol swab and let dry completely.
2. Always use bacteriostatic water (BAC) and sterile technique.
3. For a 1 mg vial, add 1 mL BAC water (creates 1 mg/mL or 1000 mcg/mL).
4. Inject water slowly down the vial wall to minimize foaming.
5. Gently roll between palms; do not shake.
6. Solution should be clear and colorless after mixing.
7. For a 100 mcg dose, draw 0.1 mL (10 units on an insulin syringe).
8. Store reconstituted peptide in the refrigerator immediately.

Quality Indicators¶

What to Expect¶

• First dose: noticeable sleep pressure and easier sleep onset
• Night 1–3: deeper sleep, fewer awakenings, vivid dreams possible
• Day 2–5: improved morning refreshment and daytime energy
• Day 5–10: cumulative stress reduction and mood improvements
• Post-cycle: benefits may persist 1–2 weeks after stopping

Side Effects & Safety¶

• Generally well tolerated with minimal side effects in reported series
• Possible mild drowsiness or dizziness initially
• Occasional headaches reported in sensitive individuals
• No tolerance or dependence reported in studies to date
• Avoid driving until individual response is known
• Not recommended during pregnancy or breastfeeding

Quick Start¶

• Typical dose: 100–200 mcg per injection
• How often: Once daily, 30–60 minutes before bed
• Where to inject: Subcutaneous — abdomen, thigh, upper arm
• Storage: Refrigerate 2–8°C; protect from light; use within 14 days
• Cycle length: 5–10 consecutive days; 2–4 week break between cycles

References¶