How To Use Peptides¶
The entire process is only 3 steps
1. Mix → 2. Draw → 3. Inject
After 2–3 tries it takes less than 60 seconds and becomes completely automatic.
Step 1: Reconstitute the Peptide¶
Reconstitution – Do it once per vial
- You receive a small glass vial with freeze-dried white (or slightly yellow) powder.
- Use bacteriostatic water (BAC water) → never regular water or saline.
- Wipe both rubber tops with an alcohol swab.
- Draw 1–2 ml of BAC water with a large syringe (3 ml is fine).
- Slowly drip the water down the inside wall of the vial → try to not blast the powder directly. But if you accidentally push the water in the powder, that's also okay.
- Gently roll or swirl the vial until fully dissolved (usually 1–2 minutes).
Never shake vigorously → it can damage the peptide chain. - Write the mixing date on the vial.
- Store in the refrigerator door (2–8 °C). Good for 4–6 weeks.
Step 2: Draw the Correct Dose¶
Always use a peptide calculator
- Use insulin syringes (0.3 ml, 0.5 ml or 1 ml – 31G or 30G is almost painless).
- Popular free calculators: Peptide Calculator or Peptide Calculator
Step 3: Inject Subcutaneously (Fat Layer)¶
Easiest & safest method
Best sites (rotate daily):
- Belly fat (2 inches away from navel)
- Love handles
- Front or outer thigh
- Back of upper arm (if someone helps)
How to inject:
- Clean skin with alcohol swab.
- Pinch 2–3 inches of fat.
- Insert needle quickly at 90°.
- Push plunger slowly (3–5 seconds).
- Pull out → no need to rub.
- Dispose syringe in a thick plastic bottle, ice cream container, or sharps container.
When Should I Inject?¶
Timing depends on the peptide
| Peptide type | Best time |
|---|---|
| BPC-157, TB-500 | Daily, morning or night |
| Tirzepatide / Retatrutide / Semaglutide | Same day every week |
| GHK-Cu (skin) | Daily, morning or night |
Detailed timing for every peptide is in its own guide → check the Peptides section.
You’ve got this!
After your first week you’ll do the whole process with your eyes closed.
Start slow, stay consistent, and always listen to your body.